Porcine FLT3L/FMS Like Tyrosine Kinase 3 Ligand ELISA Kit

Assay Genie offers a Sandwich ELISA, Double Antibody format designed for the quantitative detection of FLT3L (FMS-like tyrosine kinase 3 ligand) in porcine samples. The colorimetric assay is compatible with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and the kit has a stated sensitivity of 18.75 pg/mL with a working range of 31.25-2000 pg/mL. Reagents are intended to be stored at 2-8°C for 12 months.

Linearity has been evaluated for serum (86-99% at 1:2, 90-105% at 1:4, 85-98% at 1:8, n=5), EDTA plasma (82-101% at 1:2, 82-101% at 1:4, 82-93% at 1:8, n=5) and heparin plasma (87-97% at 1:2, 83-94% at 1:4, 88-100% at 1:8, n=5). Recovery data are reported for serum (range 89-102%, average 94%, n=5), EDTA plasma (range 86-103%, average 95%, n=5) and heparin plasma (range 87-99%, average 94%, n=5).

The protocol follows a six-step procedure covering reagent and plate preparation at room temperature, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody incubation at 37°C for 60 minutes, HRP-Streptavidin (SABC) binding at 37°C for 30 minutes, TMB color development in the dark for 10-20 minutes, and stop reaction with absorbance reading at 450 nm. Sample-specific preparation guidance is provided for serum, plasma, tissue homogenate, cell culture supernatant and cell lysate, with technical support available for additional sample types.