Porcine F1+2/Prothrombin Fragment 1+2 ELISA Kit

From Assay Genie, this Porcine F1+2/Prothrombin Fragment 1+2 ELISA Kit is a Sandwich ELISA built on a double-antibody format for measuring prothrombin fragment 1+2 levels in porcine samples. Prothrombin fragment 1-2 is described as a key marker of blood coagulation and thrombosis, making the kit useful for research in hemostasis, cardiovascular diseases, and clotting disorders. The assay is reported to be highly sensitive and specific, and accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

The kit has a stated sensitivity of 3.75 ng/mL and a measuring range of 6.25-400 ng/mL, with a colorimetric read-out based on TMB substrate development and absorbance measurement at 450 nm. Reported average recoveries are 96% for serum, 95% for EDTA plasma, and 96% for heparin plasma. Linearity has been evaluated at 1:2, 1:4, and 1:8 dilutions across the same matrices. Unopened kit components are stored at 2-8°C for up to 12 months.