Porcine ELANE (Neutrophil Elastase) ELISA Kit (PRFI00238)

Neutrophil elastase (NE) is a serine protease stored in the azurophilic granules of porcine neutrophils, where it participates in pathogen clearance and extracellular matrix remodeling during inflammation. The Assay Genie Porcine ELANE ELISA Kit provides an in vitro quantitative immunoassay for measuring neutrophil elastase in research specimens, with all reagents supplied in a single kit configured for 96 Assays. Intended for research use only, the assay supports studies in swine models of acute lung injury, sepsis, and neutrophil-mediated tissue damage, where circulating elastase serves as a marker of neutrophil activation and degranulation. Reconstituted standards and working solutions should be refrigerated between uses, and kit components should be protected from light to preserve signal stability. Each kit includes a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate, and stop solution, allowing laboratories to generate standard curves from spiked porcine samples without specialized equipment beyond a standard plate reader.