Porcine Complement C3/C3 ELISA Kit

This Sandwich ELISA from Assay Genie is designed for the quantitative measurement of Complement Component 3 (C3) in porcine serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The Porcine Complement C3/C3 ELISA Kit employs a double-antibody format with colorimetric detection, delivering a sensitivity of 0.141 ng/mL and a working range of 0.234-15 ng/mL. Storage is at 2-8°C for 12 months, and the product is supplied in a solid-phase format that ships at +4°C.

Manufacturer data report recovery in serum (n=5) of 86-102% (average 95%), EDTA plasma (n=5) of 89-98% (average 93%), and heparin plasma (n=5) of 87-103% (average 94%). Linearity at dilutions 1:2, 1:4 and 1:8 is given as 85-102%, 90-96% and 87-104% for serum, 88-101%, 83-101% and 83-97% for EDTA plasma, and 80-97%, 80-98% and 80-90% for heparin plasma. The procedure involves equilibrating reagents and TMB substrate to room temperature, incubating standards and samples at 37°C for 90 minutes, adding biotin-labelled detection antibody for 60 minutes, then HRP-streptavidin for 30 minutes, developing colour in the dark for 10-20 minutes, and reading absorbance at 450 nm. Complement C3 is described as a key component of the immune system, central to the complement cascade and pathogen clearance, with dysregulation relevant to inflammatory and autoimmune disease research.