Porcine CNTF/Ciliary neurotrophic factor ELISA Kit

Assay Genie provides a high-quality Sandwich ELISA for the quantitative measurement of Ciliary Neurotrophic Factor (CNTF) in porcine samples. The assay is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. CNTF is a vital neurotrophic factor that supports the growth, survival, and differentiation of neuronal cells, and its involvement in various neurological disorders and conditions makes it a key biomarker for neurodegenerative disease research.

Performance specifications include a sensitivity of 37.5 pg/mL and a detection range of 62.5-4000 pg/mL, with a colorimetric detection method. Linearity validation across serum, EDTA plasma, and heparin plasma samples demonstrates consistent results at 1:2, 1:4, and 1:8 dilution factors, with ranges between 83-105% depending on sample type and dilution. Recovery rates average between 91-96% across the same sample matrices, with ranges spanning 87-106%.

The assay procedure follows a standard sandwich ELISA workflow: reagent and plate preparation, primary incubation at 37°C for 90 minutes, detection antibody binding for 60 minutes, HRP-streptavidin binding for 30 minutes, TMB substrate color development in the dark for 10-20 minutes, and stop reaction with absorbance measurement at 450 nm. Sample preparation protocols are tailored for each specimen type, and the kit includes pre-coated microplate, lyophilized standard, biotin-labeled antibody, HRP-streptavidin conjugate, TMB substrate, dilution buffers, wash buffer, and stop solution, stored at 2-8°C for 12 months.