Porcine CD14 ELISA Kit

Assay Genie supplies a Sandwich ELISA kit for the quantitative detection of CD14 in porcine samples. The assay uses a Double Antibody Sandwich configuration and is validated for reactivity with porcine (swine) specimens. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Reported performance figures include a sensitivity of 0.469 ng/mL and a working range of 0.781–50 ng/mL. Linearity across serum, EDTA plasma, and heparin plasma (n = 5 per matrix) sits between 82% and 104% at 1:2, 1:4, and 1:8 dilutions. Recovery averages 93% in serum, 95% in EDTA plasma, and 94% in heparin plasma, with individual ranges of 86–99%, 87–105%, and 86–102% respectively. The protocol employs a biotin-labelled detection antibody, an HRP-Streptavidin conjugate, and TMB substrate; absorbance is read at 450 nm following a stop step. Reagents are stored at 2–8°C for 12 months.