Porcine Cadherin, Epithelial (CDHE) ELISA Kit

The Porcine Cadherin, Epithelial (CDHE) ELISA Kit from Assay Genie enables quantitative measurement of E-cadherin (CDH1, uvomorulin), a calcium-dependent transmembrane glycoprotein that mediates homotypic adhesion between epithelial cells. Each kit contains a pre-coated microplate, lyophilized standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate, and stop solution, sufficient for one full plate of quantitative assays.

E-cadherin is a central regulator of adherens junctions and epithelial barrier integrity, and reduced CDH1 expression is associated with epithelial-mesenchymal transition (EMT), tumor cell invasion, and metastatic dissemination in gastric, breast, and colorectal carcinomas. Researchers apply this kit in porcine models to study gastrointestinal physiology, intestinal barrier function, reproductive biology, wound healing, and xenograft cancer research. The capture antibody is specific to the extracellular domain of porcine E-cadherin and shows no significant cross-reactivity with N-cadherin or P-cadherin, allowing accurate measurement in tissue homogenates, cell lysates, and cell culture supernatants. Store standards and detection antibody aliquots at -20 degrees C; bring all reagents to room temperature before use, and avoid repeated freeze-thaw cycles of the reconstituted standard.