Porcine Apolipoprotein E (APOE) ELISA Kit

The Porcine Apolipoprotein E (APOE) ELISA Kit from Assay Genie uses a sandwich ELISA format with colorimetric detection at 450 nm to quantify APOE in serum and plasma samples. APOE is a ~34 kDa glycoprotein that mediates cholesterol transport and lipid metabolism, and the porcine ortholog is widely used as a translational model for cardiovascular and atherosclerosis research. Each kit contains a pre-coated 96-well microplate, biotinylated detection antibody, streptavidin-HRP conjugate, standards, and assay diluent sufficient for 96 determinations. The assay demonstrates low cross-reactivity with related apolipoproteins and supports a quantitative dynamic range across physiological and elevated APOE concentrations. Store all reagents at 2-8 °C between uses, avoid repeated freeze-thaw cycles of reconstituted standards, and equilibrate components to room temperature before running the protocol. The complete workflow typically requires 3-4 hours from sample loading to readout, supporting routine in vitro quantification in swine model studies of lipid disorders, metabolic syndrome, and dietary intervention.

For optimal data quality, run all samples in duplicate and generate a fresh seven-point standard curve on each plate spanning roughly 0.156–10 ng/mL to enable accurate interpolation of unknown values. The capture antibody is pre-immobilized on the microplate wells, removing the need for overnight coating and shortening hands-on time. Porcine serum and EDTA-plasma samples typically perform best at 1:200 to 1:500 dilution in the supplied assay diluent, while heparinized plasma may introduce optical interference and should be avoided where possible. Reconstitute lyophilized standards with the provided buffer immediately before use, and discard any aliquots that have undergone more than two freeze-thaw cycles to preserve immunoreactivity.

Typical intra-assay coefficient of variation falls below 8% and inter-assay CV remains under 12% when the protocol is followed precisely. Assay Genie supplies all necessary reagents calibrated against purified porcine APOE reference material, and the kit supports translational workflows in Göttingen minipig models of familial hypercholesterolemia, Ossabaw swine studies of metabolic syndrome, and Yucatan pig dietary intervention trials. Researchers routinely correlate circulating APOE measurements with hepatic VLDL output, aortic lipid staining, and LDL receptor expression in downstream western blot and qPCR assays, making this ELISA a practical anchor for integrated cardiovascular phenotyping pipelines.