Porcine Apolipoprotein E/Apo-E ELISA Kit

This Sandwich ELISA kit from Assay Genie is described as a highly sensitive and specific assay for the accurate detection of Apolipoprotein E (Apo E) in porcine samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The double-antibody format supports research applications in lipid metabolism, cholesterol transport and clearance, and the study of cardiovascular diseases and metabolic disorders in pigs.

Reported performance characteristics include a sensitivity of 0.75 ng/mL and a working range of 1.25–80 ng/mL. Linearity across 1:2, 1:4 and 1:8 dilutions falls between 81% and 103% for serum, EDTA plasma and heparin plasma samples (n = 5). Recovery ranges from 85% to 105% with average values of 93% for serum, 95% for EDTA plasma and 95% for heparin plasma. Unopened components are stored at 2–8°C and the kit remains stable for 12 months under those conditions.

The workflow uses a biotinylated detection antibody with HRP-Streptavidin (SABC) and a TMB substrate, with key incubations carried out at 37°C, and absorbance read at 450 nm after addition of stop solution. Listed protein synonyms include APOE, Apolipoprotein E, Apo-E, MGC1571, and apolipoprotein E3 (UniProt P18650).