Porcine Apolipoprotein A-I-binding protein (APOA1BP) ELISA Kit

This colorimetric sandwich ELISA from Assay Genie enables quantitative measurement of porcine APOA1BP in serum and plasma samples, supporting investigations into lipoprotein metabolism, reverse cholesterol transport, and cardiovascular disease biology. The 96-well microplate format supplies pre-coated capture plates, lyophilized standards, biotinylated detection antibody, and streptavidin-HRP conjugate for construction of a standard curve and interpolation of unknown sample concentrations.

Typical workflow involves sample dilution, sequential incubation steps with washing between each, TMB substrate addition, and absorbance readout at 450 nm on a standard plate reader. Reconstituted reagents should be aliquoted and stored according to the insert; unused strips may be refrigerated in sealed pouches with desiccant. Intended for research use only, not for diagnostic or therapeutic applications.

APOA1BP, also termed ApoA-I binding protein or AIBP, participates in HDL biogenesis by accelerating cholesterol efflux through the ABCA1 and ABCG1 transporters, and recent work has expanded its role into inflammation control, hematopoietic stem cell maintenance, and neural tube patterning. Quantifying circulating APOA1BP in swine models therefore supports translational cardiovascular research, metabolic syndrome studies, and investigations of lipid-driven neuroinflammation where pigs serve as a physiological surrogate for humans.

Each kit lot is shipped at 2-8 °C; reconstituted standard and biotinylated detection antibody should be aliquoted and held at -20 °C with limited freeze-thaw cycles, while the sealed microplate pouch remains stable at 4 °C through the printed expiration. The assay accommodates 50 ul of diluted serum or plasma per well, producing a working dynamic range near 0.16-10 ng/mL with intra-assay CV typically below 10%. A run from sample loading to final absorbance at 450 nm generally completes within 3.5 hours, and Assay Genie supplies a protocol insert with recommended dilution factors for hyperlipidemic porcine samples.