Porcine Alkaline Phosphatase/ALPL ELISA Kit

A double-antibody Sandwich ELISA from Assay Genie for the quantification of Alkaline Phosphatase (ALPL) in porcine samples, using colorimetric detection. Sensitivity is 0.938 ng/mL with a measuring range of 1.563–100 ng/mL. The assay is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types, with reagents stored at 2–8°C for up to 12 months.

ALPL, also referred to as ALP or Alkaline Phosphatase, plays a key role in biological processes including bone mineralization, cell growth, and signalling pathways. Dysregulation of ALPL has been associated with bone disorders, liver disease and other health conditions, making it a valuable biomarker for research into these diseases.

Across the three serum/plasma matrices, average recovery is 97% (serum), 95% (EDTA plasma) and 93% (heparin plasma), with linearity between 82% and 104% across 1:2, 1:4 and 1:8 dilutions (n = 5 per matrix).