PLK1 Colorimetric Cell-Based ELISA Kit

Assay Genie offers this Cell-Based ELISA Kit designed for the quantitative measurement of PLK1 protein levels directly in cultured cells without the need for lysate preparation. The kit provides a high-throughput, sensitive workflow for measuring the relative amounts of PLK1 in cultured cells and for screening the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on PLK1 expression. It is intended for adherent cell samples and shows reactivity with Human, Mouse, and Rat.

Detection is achieved through an indirect ELISA format in which PLK1 is captured by PLK1-specific primary antibodies while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, yielding a colorimetric readout at 450 nm. The stated dynamic range is > 5000 Cells. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing target absorbance values, and a Crystal Violet whole-cell staining method is provided to determine cell density so that absorbance values can be adjusted for plating differences.

PLK1 (Polo-like kinase 1) is a key regulator of cell cycle progression and cytokinesis and is overexpressed in many cancer types, making it a target of interest in cancer research. The PLK1 gene maps to chromosomal location 16p12.2 (Gene ID: 5347; UniProt ID: P53350). According to the manufacturer's UniProt entry, PLK1 belongs to the PLK family of kinases and contains a polo-box domain that binds specific phosphoserine or phosphothreonine residues, with listed substrates including BRCA2, Myt1, NudC, Cdc25C, cyclin B1, and Nlp. The kit is stored at 4 °C with a stated stability of 6 Months.