PLCG2 (Phospho-Tyr753)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the qualitative detection of PLCG2 phosphorylation at tyrosine 753 in cultured adherent cells, using an indirect colorimetric ELISA format read at 450 nm. The kit reacts with human, mouse and rat samples and supports a dynamic range of > 5000 Cells. Its lysate-free workflow captures the phospho-target with target-specific primary antibodies, with HRP-conjugated secondary antibodies providing the colorimetric readout upon substrate addition.

Because the assay is qualitative, two normalization strategies are included: a monoclonal anti-GAPDH antibody serves as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining method can be used to determine cell density so that absorbance values can be adjusted to cell amount and plating differences accounted for.

PLCG2 is a calcium-dependent phosphatidylinositol-specific phospholipase C that acts as a crucial enzyme in transmembrane signaling, generating the second messengers diacylglycerol and inositol 1,4,5-trisphosphate. The kit can be used to measure the relative amount of phosphorylated PLCG2 in cultured cells as well as to screen the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on PLCG2 phosphorylation.