PLCG1 (Phospho-Tyr783)Fluorometric Cell-Based ELISA Kit

From Assay Genie, this Phospho Cell-Based ELISA Kit is designed to monitor PLCG1 phosphorylation and expression profiles in cultured cells. It provides a convenient, lysate-free, high-throughput and sensitive workflow for measuring the relative amounts of phosphorylated PLCG1 (Tyr783) in cells, and can be used to screen the effects of various treatments, inhibitors such as siRNA or chemicals, and activators on PLCG1 phosphorylation.

The kit uses an indirect ELISA format in which phospho-PLCG1 (Tyr783) is captured by target-specific primary antibodies, while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody to enable fluorometric detection. Two normalization strategies are included: a monoclonal antibody specific for human GAPDH serves as an internal positive control for normalizing target RFU values, and an antibody against the non-phosphorylated counterpart of PLCG1 (Tyr783) is provided to normalize the RFU value for phosphorylated PLCG1.

Reactivity covers Human, Mouse and Rat samples, with a dynamic range of > 5000 cells, fluorometric detection and a Cell-Based ELISA format. The assay is read using a fluorescent plate reader at Ex/Em 651/667 (Dye 1) and 495/521 (Dye 2), and the kit is stored at 4°C with 6 months stability.