PLCG1 (Phospho-Tyr783)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the detection of phosphorylated PLCG1 at tyrosine 783 in cell lysates and tissue homogenates, using adherent cells. The assay uses a colorimetric detection method at 450 nm and follows an indirect ELISA format, in which the phospho-target is captured by PLCG1 (Phospho-Tyr783)-specific primary antibodies and detected using HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition.

The kit has been validated for reactivity with human, mouse and rat samples and has a stated dynamic range of > 5000 Cells. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and the Crystal Violet whole-cell staining method can be used to determine cell density, so that absorbance readings can be normalized to cell amounts and plating differences adjusted.

PLCG1 is described as a calcium-dependent phosphatidylinositol-specific phospholipase C that produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate, and phosphorylation at tyrosine 783 is known to modulate its enzymatic activity and downstream signaling cascades associated with cell growth, differentiation and immune responses.