PLCbeta3 (Phospho-Ser537)Colorimetric Cell-Based ELISA Kit

A colorimetric cell-based ELISA kit from Assay Genie is designed for the accurate measurement of phosphorylated serine 537 levels in PLCbeta3 in cell lysates. The assay uses an indirect ELISA format to detect PLCbeta3 protein phosphorylation and expression profiles in cells, making it suitable for cell biology and drug discovery research. The dynamic range is > 5000 cells with detection measured at 450 nm.

Reactivity is confirmed for human, mouse, and rat samples, with adherent cells as the sample type. The kit is lysate-free and high throughput. PLCbeta3 (phospholipase C beta 3, UniProt ID: Q01970) is a phosphatidylinositol-specific phospholipase C that binds calmodulin and produces the second messengers diacylglycerol and inositol 1,4,5-trisphosphate. The protein has a molecular weight of 131,205 Da. Phosphorylation of serine 537 is a key event in cellular signaling pathways regulating cell growth, differentiation, and proliferation, and its dysregulation has been implicated in cancer, diabetes, and neurological disorders.

Normalization is achieved through a monoclonal antibody specific for human GAPDH as an internal positive control, and Crystal Violet whole-cell staining is used to determine cell density. The kit can be used to screen for the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on PLCbeta3 phosphorylation.