PLCbeta3 Colorimetric Cell-Based ELISA Kit

Designed for the precise measurement of PLCbeta3 in cell samples, this Colorimetric Cell-Based ELISA Kit from Assay Genie offers reactivity with Human, Mouse, and Rat and a colorimetric detection method at 450 nm. The cell-based, lysate-free assay has a dynamic range of > 5000 Cells, supporting high-throughput screening of PLCbeta3 protein expression in cultured cells, and can be used to evaluate the effects of various treatments, inhibitors, or activators on the target.

Qualitative determination of PLCbeta3 concentration is achieved via an indirect ELISA format: PLCbeta3-specific primary antibodies capture the target, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values. After the colorimetric measurement, a Crystal Violet whole-cell staining step may be used to determine cell density, with results analysed by normalizing absorbance values to cell amounts to adjust for plating differences.

PLCB3 is a phosphatidylinositol-specific phospholipase C that binds calmodulin and, when activated, produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate. The gene is identified by Gene ID 5331 and UniProt ID Q01970, encoding a protein of 131,205 Da. The kit is stored at 4°C with a 6-month stability and falls within the metabolism research area.