PKCB (Phospho-Ser661)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit is designed to detect phosphorylation of PKCΒ at Ser661 in cell samples, supporting research in cell signaling and protein phosphorylation studies. The assay uses a colorimetric detection method measured at 450 nm in an indirect ELISA format, with HRP-conjugated secondary antibodies producing a colorimetric reaction upon substrate addition. The kit is lysate-free, high throughput, and intended for qualitative measurement of phosphorylated PKCΒ in cultured cells, as well as screening the effects of treatments, inhibitors (e.g. siRNA or chemicals), or activators on PKCΒ phosphorylation.

The kit is reactive with Human, Mouse, and Rat samples, with a stated dynamic range of > 5000 Cells. Normalization is supported by an included anti-GAPDH monoclonal antibody serving as an internal positive control, and by Crystal Violet whole-cell staining to determine cell density after colorimetric measurement. The target is the PRKCB gene product (Gene ID: 5579; UniProt ID: P05771; OMIM: 176970), and the sub-type is Phospho.

Suitable for adherent cell samples, the kit is relevant to studies of PKCΒ's role in cellular processes such as cell growth, differentiation, and apoptosis, with implications in diseases including cancer, diabetes, and cardiovascular disorders.