PKC zeta (Phospho-Thr410)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA kit offers a sensitive, high-throughput and lysate-free workflow for studying the phosphorylation status of PKC zeta at Thr410. It enables qualitative determination of phosphorylated PKC zeta in cultured cells through an indirect ELISA format, and can be used to screen the effects of treatments, inhibitors such as siRNA or chemicals, and activators on PKC zeta phosphorylation.

The assay uses a colorimetric readout at 450 nm and is reactive with Human, Mouse and Rat samples. It provides a dynamic range of > 5000 Cells and is designed for use with adherent cell cultures. The target corresponds to gene PRKCZ (Gene ID: 5590; UniProt ID: Q05513), with a phospho-specific sub-type and applications in immunology research.

Detection relies on PKC zeta (Phospho-Thr410)-specific capture antibodies together with HRP-conjugated secondary antibodies. An anti-GAPDH monoclonal antibody is included as an internal positive control for normalizing target absorbance, and Crystal Violet whole-cell staining is provided for determining cell density so that plating differences can be adjusted.