PKC delta (Phospho-Tyr52)Colorimetric Cell-Based ELISA Kit

Assay Genie's colorimetric Cell-Based ELISA Kit targets phosphorylated PKC delta at tyrosine 52, providing a lysate-free, sensitive, high-throughput assay for quantifying phosphorylation status in cell samples. The kit is reactive with Human, Mouse, and Rat samples and is designed for adherent cells, with a stated dynamic range of "> 5000 Cells" and detection by colorimetric measurement at 450 nm.

Qualitative determination is achieved through an indirect ELISA format in which the target is captured by phospho-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, enabling a colorimetric reaction upon substrate addition. The kit can be used to measure relative levels of phosphorylated PKC delta in cultured cells and to screen for the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on PKC delta phosphorylation. For normalization, an anti-GAPDH monoclonal antibody is included as an internal positive control, and Crystal Violet whole-cell staining may be used to determine cell density.

Associated database identifiers include Gene ID 5580, UniProt ID Q05655, OMIM 176977, and Unigene Hs.155342. The gene symbol is PRKCD, the sub-type is Phospho, and the molecular weight is reported as 80,953 Da.