PKA-R2beta Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA kit is designed for the qualitative detection of PKA-R2beta, a regulatory subunit of cAMP-dependent protein kinase (PRKAR2B), in cultured cells. The colorimetric assay is read at 450 nm and is reactive with human, mouse and rat samples, supporting research into cell signalling pathways and protein phosphorylation events.

The lysate-free, high-throughput workflow uses an indirect ELISA format in which PKA-R2beta-specific primary antibodies capture the target, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyse a colorimetric reaction upon substrate addition. It can be used to measure relative PKA-R2beta amounts in cultured cells and to screen the effects of treatments, inhibitors (such as siRNA or chemicals) or activators on PKA-R2beta expression.

Two normalisation strategies are included: a monoclonal antibody specific for human GAPDH serves as an internal positive control, and Crystal Violet whole-cell staining is used to determine cell density after the colorimetric read. Quoted performance figures include a dynamic range of greater than 5,000 cells and storage stability of 4 °C for 6 months.