PITX1 Colorimetric Cell-Based ELISA

Assay Genie's Cell-Based ELISA Kit is specifically designed for the detection of PITX1 levels in cell lysates and culture supernatants, using adherent cells as the sample type. The assay uses a colorimetric detection method read at 450 nm and is reactive with human, mouse, and rat samples. With a dynamic range of > 5000 cells and a 96-well microplate format, the kit offers a lysate-free, high-throughput workflow suitable for qualitative determination of PITX1 protein expression in cultured cells.

The indirect ELISA format relies on PITX1-specific primary antibodies, with HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. PITX1 (pituitary homeobox 1) is a transcription factor that plays a critical role in the development of various tissues and organs, including the skeletal system and limbs, and its dysregulation has been implicated in several developmental disorders, making it a valuable biomarker for research. The kit includes a monoclonal antibody specific for human GAPDH as an internal positive control for normalisation, and Crystal Violet whole-cell staining is provided to determine cell density.

The product falls within the research area of Epigenetics and Nuclear Signaling and is intended for storage at 4°C with a stated stability of 6 months.