Pim-1 (Phospho-Tyr309)Colorimetric Cell-Based ELISA Kit

A high-quality cell-based immunoassay from Assay Genie enables detection of phosphorylated PIM-1 in cell lysates through an indirect ELISA format. The colorimetric assay measures absorbance at 450 nm and is intended for the qualitative determination of Pim-1 (Phospho-Tyr309) levels in cultured cells, with demonstrated reactivity against Human, Mouse and Rat samples.

The kit is configured for adherent cell workflows and supports a dynamic range above 5000 Cells. Target capture occurs with a phospho-specific primary antibody, while an HRP-conjugated secondary antibody catalyses a colorimetric reaction upon substrate addition. Two complementary normalisation strategies are built into the workflow: a monoclonal anti-GAPDH antibody serves as an internal positive control for absorbance readings, and a Crystal Violet whole-cell staining step allows absorbance values to be normalised to cell density, compensating for plating differences. The assay is suitable for measuring the relative amounts of phosphorylated PIM-1 in cultured cells and for screening the effects of various treatments, inhibitors such as siRNA or chemicals, and activators on PIM-1 phosphorylation.

Assay Genie provides a sensitive, lysate-free, high-throughput format suited to research into PIM-1 signalling in cell proliferation, survival and differentiation. It supports studies where abnormal activation of PIM-1 has been implicated in disease, including cancer and inflammatory disorders, and is intended to aid investigation of cellular signalling pathways and potential therapeutic interventions.