Phospho-MAPK Family ELISA Sampler Kit

The Assay Genie Phospho-MAPK Family ELISA Sampler Kit bundles five separate 48-well microplate assays into one package, each calibrated to detect a distinct activated (phosphorylated) member of the mitogen-activated protein kinase cascade. The sampler targets the three principal MAPK branches — ERK1/2, JNK, and p38 — enabling researchers to compare pathway activation across the upstream kinase network in matched human samples in a single coordinated run.

Each sub-assay uses a phospho-specific capture antibody immobilised on its own 48-well plate, with an HRP and TMB substrate reaction that yields a quantitative standard curve for the activated kinase. The kit is configured for research use only, with sub-assays supplied as individually sealed microplate strips so labs can run one target at a time or all five in parallel. Store unopened components refrigerated and avoid repeated freeze-thaw cycles of the reconstituted standards to protect phospho-epitope integrity.

Typical downstream applications include dissecting MEK-ERK versus MKK4/7-JNK versus MKK3/6-p38 signalling in cancer cell signalling studies, drug-response profiling, and inflammation research, where identifying which MAPK arm is engaged is a critical first step before committing to single-target follow-up.