PHCA Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed to detect PHCA protein expression in cultured cells using an indirect colorimetric format read at 450 nm. It reacts with Human and Mouse samples and is supplied in a 96-Well Microplate format with a stated dynamic range of "> 5000 Cells" and storage at 4°C for 6 months.

The lysate-free, high-throughput workflow captures PHCA with target-specific primary antibodies, while HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance values, and Crystal Violet whole-cell staining may be used to determine cell density, allowing absorbance values to be normalized to cell amounts. The kit can be applied to measure relative PHCA amounts in cultured cells and to screen the effects of treatments, inhibitors (e.g. siRNA or chemicals) or activators.

According to the manufacturer's database information, PHCA is listed as a synonym for the gene symbol ACER3 (Gene ID: 55331; UniProt: Q9NUN7), which encodes alkaline ceramidase 3, a multi-pass integral membrane hydrolase involved in sphingolipid metabolism, including the ceramide metabolic process, sphingolipid biosynthetic process and sphingosine biosynthetic process. The stated Research Area is Metabolism and the listed Sample Type is Adherent Cells.