PEA-15 (Phospho-Ser104)Colorimetric Cell-Based ELISA Kit

This cell-based ELISA kit from Assay Genie is designed to study the phosphorylation status of PEA-15 at Ser104 in a cell-based assay format. It is a lysate-free, high throughput, and sensitive assay that detects PEA-15 protein phosphorylation and expression profiles in cells, and is listed for use with adherent cell samples. The kit can be used to measure relative amounts of phosphorylated PEA-15 in cultured cells, and to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on PEA-15 phosphorylation.

Detection is by an indirect ELISA with colorimetric readout at 450 nm. The PEA-15 (Phospho-Ser104) target is captured by phospho-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyze a colorimetric reaction on substrate addition. The kit is reactive with Human, Mouse, and Rat samples, with a dynamic range of > 5000 cells. Two normalization methods are provided: a monoclonal antibody specific for human GAPDH as an internal positive control, and a Crystal Violet whole-cell staining method to determine cell density after the colorimetric measurement.

PEA-15 (Phosphoprotein Enriched in Astrocytes 15) is a multifunctional anti-apoptotic protein involved in cell survival, proliferation, and apoptosis, and is also an endogenous substrate for protein kinase C. Phosphorylation at Ser104 has been implicated in regulating its activity, making it a valuable marker for investigating signaling pathways relevant to cancer, diabetes, and other diseases. PEA-15 is encoded by the PEA15 gene (Gene ID: 8682; UniProt ID: Q15121) and is most abundant in tissues such as heart, brain, muscle, and adipose tissue, with higher levels of expression reported in tissues from individuals with type 2 diabetes than in controls.