PDCD4 Colorimetric Cell-Based ELISA Kit

Accurate measurement of Programmed Cell Death Protein 4 (PDCD4) in cell samples is supported by this Colorimetric Cell-Based ELISA Kit from Assay Genie. The kit is designed for research applications including studies of cancer, inflammatory diseases, and other pathological conditions, as PDCD4 plays a role in cell growth regulation and apoptosis.

The assay is lysate-free and uses an indirect ELISA format. PDCD4 is captured by target-specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Substrate addition triggers a colorimetric reaction measured at 450 nm. Reactivity is reported for human, mouse, and rat samples, with a dynamic range of greater than 5000 cells. The indicated sample type is adherent cells. The kit is supplied in a 96-well microplate format and is stored at 4°C for 6 months.

For normalization, a monoclonal antibody specific for human GAPDH is included as an internal positive control. After the colorimetric measurement of HRP activity, Crystal Violet whole-cell staining can be used to determine cell density, so absorbance values can be normalized to cell amounts. Database information cited for the human target includes Gene ID 27250, UniProt ID Q53EL6, and a reported molecular weight of 50,576 Da for the PDCD4 protein.