Papain, 25 mg

A lyophilized cysteine protease derived from Carica papaya latex, STEMCELL Technologies Papain is supplied as a 25 mg vial of sterile-filtered enzyme powder intended for enzymatic tissue dissociation in primary cell isolation workflows.

Researchers reconstitute the powder in aqueous buffer to prepare working digestion solutions that break down extracellular matrix proteins in soft tissues such as brain, lung, and other organs. The enzyme releases viable cells for downstream cell culture, flow cytometry, and electrophysiology studies, with mild proteolytic activity that helps preserve cell surface epitopes and membrane integrity in sensitive neuronal preparations.

Each 25 mg vial provides enough enzyme for multiple dissociation experiments depending on tissue mass and target concentration. The lyophilized powder should be stored frozen at -20 degrees C, protected from light and moisture, to preserve enzymatic activity across the product shelf life. Working solutions are best prepared fresh and used promptly for reliable tissue digestion.