PAK1 (Phospho-Thr212)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the accurate detection of phosphorylated Pak1 at threonine 212 in cellular samples, offering high sensitivity and specificity for a variety of research applications. The assay uses a colorimetric detection method measured at 450 nm, with a stated dynamic range of > 5000 cells, and shows reactivity with human, mouse, and rat samples.

Pak1 is described as a key signaling protein involved in regulating cell growth, differentiation, and migration. Phosphorylation at threonine 212 is reported to activate Pak1, influencing cellular processes such as cytoskeletal dynamics, cell adhesion, and gene transcription. Dysregulation of Pak1 signaling has been linked to diseases including cancer, neurodegenerative disorders, and inflammatory conditions, highlighting its relevance as a therapeutic target and biomarker.

The kit is intended for adherent cell samples and follows an indirect ELISA workflow, in which the target is captured by phospho-specific primary antibodies and detected via HRP-conjugated secondary antibodies. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalization of target absorbance values, and a Crystal Violet whole-cell staining step may be used to determine cell density and adjust for plating differences.