PAK1/2/3 (Phospho-Ser144/141/139)Colorimetric Cell-Based ELISA Kit

Assay Genie's Colorimetric Cell-Based ELISA Kit is designed for the precise measurement of phosphorylated PAK1/2/3 at serine 144/141/139 levels in cell lysates. The kit targets PAK1, PAK2, and PAK3 — key regulators of cellular processes such as cell migration, cytoskeletal organization, and cell survival — and is reactive with Human, Mouse, and Rat samples.

Detection is performed by colorimetric measurement at 450 nm, with a dynamic range of > 5000 Cells. The kit is intended for use with adherent cells and provides a lysate-free, high-throughput format for measuring relative amounts of phosphorylated PAK1/2/3 in cultured cells. It can also be used to screen for the effects of various treatments, inhibitors, or activators on PAK1/2/3 phosphorylation. Qualitative determination is achieved via an indirect ELISA format, with HRP-conjugated secondary antibodies catalysing a colorimetric reaction upon substrate addition.

Normalization is supported by an anti-GAPDH monoclonal antibody included as an internal positive control, together with a Crystal Violet whole-cell staining method to determine cell density. Disruption of PAK signalling has been implicated in various diseases including cancer, neurological disorders, and immune disorders, making the kit a valuable tool for studying PAK signalling pathways and investigating potential therapeutic targets.