p90 RSK (Phospho-Thr359+Ser363)Colorimetric Cell-Based ELISA Kit

Assay Genie offers a colorimetric, cell-based ELISA kit for detecting phosphorylation of p90 RSK at Thr359 and Ser363 directly in cultured cells, without the need to prepare lysates. Designed as a high-throughput and sensitive assay, the kit uses an indirect ELISA format in which target-specific primary antibodies capture p90 RSK (Phospho-Thr359+Ser363), and HRP-conjugated secondary antibodies bound to the Fc region of the primary antibody catalyze a colorimetric reaction upon substrate addition, measured at 450 nm.

The kit is reactive with human, mouse, and rat samples and is intended for use with adherent cells, supporting measurement of the relative levels of phosphorylated p90 RSK as well as screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on p90 RSK phosphorylation. Because the assay is qualitative, two normalization strategies are incorporated: a monoclonal anti-GAPDH antibody acts as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining method is used to determine cell density so that absorbance values can be adjusted for plating differences.

p90 RSK is described as a key signaling protein involved in cell growth, proliferation, and survival, and abnormal phosphorylation of sites including Thr359 and Ser363 has been linked to cancer, inflammatory disorders, and metabolic diseases, making this phosphorylation profile an important target for research and drug development. According to the manufacturer's specification, the dynamic range is > 5000 cells.