p90 RSK Colorimetric Cell-Based ELISA Kit

Assay Genie offers a colorimetric cell-based ELISA kit for the detection of p90 RSK, a protein kinase involved in cellular processes such as cell growth, differentiation, and survival. The kit is designed for the accurate and reliable measurement of p90 RSK levels, with reactivity against human, mouse, and rat samples, and is validated for use with adherent cells. It provides a high-throughput, lysate-free workflow suitable for studying the relative amounts of p90 RSK in cultured cells, as well as for screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on p90 RSK expression.

Detection is achieved through an indirect ELISA format measured at 450 nm, using p90 RSK-specific primary antibodies and HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalization of target absorbance values. Following the colorimetric measurement, the Crystal Violet whole-cell staining method may be used to determine cell density, allowing absorbance values to be normalized to cell amounts and plating differences to be adjusted. The kit has a dynamic range of greater than 5000 cells, is provided in a microplate format, and is intended for research in the cell cycle area. Reagents should be stored at 4°C with a stability of 6 months.

Dysregulation of p90 RSK has been linked to cancer, cardiovascular diseases, and neurological disorders, highlighting its importance as a biomarker and its value in studies of disease progression and potential therapeutic interventions. The associated gene is RPS6KA1 (Gene ID: 6195; UniProt ID: Q15418).