p70 S6 Kinase (Phospho-Thr229)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA is designed for the detection of phosphorylated p70 S6 Kinase at threonine 229, supporting research into cell signaling pathways and protein phosphorylation dynamics related to cell growth, proliferation, and metabolism.

The assay uses a colorimetric method with measurement at 450 nm in an indirect ELISA format, in which p70 S6 Kinase (Phospho-Thr229) is captured by specific primary antibodies and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyze the colorimetric reaction. It is suitable for adherent cells and shows reactivity with human, mouse, and rat samples, with a stated dynamic range of > 5000 Cells. A monoclonal antibody specific for human GAPDH is included as an internal positive control, and the Crystal Violet whole-cell staining method may be used to determine cell density for normalization.

Because the Cell-Based ELISA is qualitative, the kit enables measurement of relative phosphorylation levels in cultured cells as well as screening for the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on p70 S6 Kinase phosphorylation, supporting research areas including cancer biology, metabolic disorders, cellular responses to external signals, and cell death.