p70 S6 Kinase (Phospho-Ser424)Fluorometric Cell-Based ELISA Kit

From Assay Genie, this Phospho Cell-Based ELISA Kit provides qualitative detection of phosphorylated p70 S6 Kinase at Ser424 directly in cultured cells. The kit is lysate-free, high-throughput and sensitive, allowing researchers to monitor p70 S6 Kinase protein phosphorylation and expression profiles and to measure the relative amounts of phosphorylated p70 S6 Kinase in cultured cells. It is also suited to screening the effects that various treatments, inhibitors (such as siRNA or chemicals) or activators have on RPS6KB1 phosphorylation. Reactivity is established for Human, Mouse and Rat samples.

Detection uses an indirect ELISA format: the target is captured by phospho-p70 S6 Kinase (Ser424)-specific primary antibodies, while Dye 1- and Dye 2-conjugated secondary antibodies bind the Fc region of the primaries, enabling a fluorometric readout. Plates are read at Ex/Em of 651/667 (Dye 1) and 495/521 (Dye 2). The dynamic range is greater than 5000 cells, and recommended storage is 4 °C with a stability of 6 months.

Two normalization reagents are included: a monoclonal antibody specific for human GAPDH acts as an internal positive control for normalizing target RFU values, and an antibody against the non-phosphorylated counterpart of p70 S6 Kinase (Ser424) enables normalization against total p70 S6 Kinase RFU values. The associated NCBI Gene Symbol is RPS6KB1 (Gene ID 6198; UniProt ID P23443; OMIM 608938; Unigene Hs.463642).