p70 S6 Kinase (Phospho-Ser424)Colorimetric Cell-Based ELISA Kit

A cell-based ELISA kit from Assay Genie provides a sensitive, lysate-free, high-throughput method for detecting phospho-p70 S6 Kinase at Ser424 directly in cultured adherent cells. The indirect ELISA format uses target-specific primary antibodies to capture the phosphorylated kinase, while HRP-conjugated secondary antibodies catalyze a colorimetric reaction measured at 450 nm. The assay can be used to measure relative phosphorylation levels in cells and to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on p70 S6 Kinase signaling.

Validated for human, mouse, and rat samples, the kit has a dynamic range of > 5000 Cells. Because the Cell-Based ELISA is qualitative, two normalization methods are provided: a monoclonal anti-human GAPDH antibody serves as an internal positive control for normalizing target absorbance, and Crystal Violet whole-cell staining determines cell density so absorbance values can be adjusted for plating differences. The target is encoded by the RPS6KB1 gene (Gene ID 6198, UniProt P23443) and belongs to the AGC kinase family, with activity required for cell growth and G1 cell cycle progression downstream of mTOR signaling.