p70 S6 Kinase Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for detecting and quantifying p70 S6 Kinase in cell lysates using an indirect colorimetric detection method measured at 450 nm. The kit is reactive with human, mouse, and rat samples and supports a dynamic range of greater than 5000 cells. It is supplied in a 96-well microplate format and is intended for use with adherent cell samples.

The assay is a convenient, high-throughput, and sensitive procedure that operates without the need for lysate preparation. p70 S6 Kinase-specific primary antibodies capture the target, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values, and Crystal Violet whole-cell staining may be used to determine cell density and account for plating differences. The kit can be applied to measure relative levels of p70 S6 Kinase in cultured cells and to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on p70 S6 Kinase expression.

p70 S6 Kinase is a key regulator of cell growth, proliferation, and protein synthesis, and dysregulation of this kinase has been associated with conditions such as cancer, diabetes, and inflammatory diseases. The kit is stored at 4 °C with a stated stability of 6 months.