p63 Transcription Factor Activity Assay

Supplied by Assay Genie, this 96 Assays transcription factor activity kit is intended for research use only and shipped on cold packs to preserve antibody and oligonucleotide integrity. The kit contains a pre-coated microwell strip plate, p63-specific primary antibody, HRP conjugate, wash buffer, and developing substrate. Working antibody dilutions are stored short-term at 4 degrees C, while reconstituted reagents should be aliquoted and held at -20 degrees C to limit freeze-thaw degradation across the stated shelf life. For consistent quantification, load 5 to 15 micrograms of nuclear protein per well; lysates prepared with protease and phosphatase inhibitors give the cleanest signal-to-background ratio. The format complements chromatin immunoprecipitation, luciferase reporter assays, and immunofluorescence readouts of TAp63 and DeltaNp63 isoform activity in epithelial, keratinocyte, squamous carcinoma, and triple-negative breast cancer models, supporting translational drug screening and developmental biology studies in human, mouse, and rat samples.