p62 Dok (Phospho-Tyr398)Colorimetric Cell-Based ELISA Kit

This Colorimetric Cell-Based ELISA Kit from Assay Genie is designed to detect phosphorylation of p62 Dok (Phospho-Tyr398) in cell-based assays, providing a sensitive and specific tool for studying this modification. The p62/DOK protein functions as a key adaptor involved in cell signalling and autophagy, and phosphorylation at Tyr398 has been implicated in diseases such as cancer, inflammation, and neurodegenerative disorders.

Detection is achieved by an indirect ELISA format, in which HRP-conjugated secondary antibodies catalyse a colorimetric reaction read at 450 nm. The assay is described as convenient, lysate-free, high throughput, and sensitive, and is intended for use with adherent cell samples. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalising target absorbance, and Crystal Violet whole-cell staining can be applied to determine cell density. The kit is reactive with Human, Mouse, and Rat samples, and has a stated dynamic range of > 5000 Cells.