p56 Dok-2 Colorimetric Cell-Based ELISA Kit

Manufactured by Assay Genie, this Cell-Based ELISA Kit is designed for the qualitative detection of p56 Dok-2 protein expression in cultured cells. The kit uses a colorimetric detection method at 450 nm in a 96-Well Microplate format and is reactive with Human and Mouse samples, with a dynamic range of > 5000 Cells. Intended for use with Adherent Cells, it provides a convenient, lysate-free, high-throughput approach for measuring relative p56 Dok-2 levels and for screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on p56 Dok-2 expression.

The assay employs an indirect ELISA format in which p56 Dok-2 is captured by specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values, and Crystal Violet whole-cell staining may be used to determine cell density, allowing absorbance values to be normalised to cell amounts to adjust for plating differences. The kit is stored at 4°C with a stated stability of 6 Months and falls within the Immunology research area.