p53 (Phospho-Thr18)Transcription Factor Activity Assay

The p53 protein is a critical tumor suppressor and master regulator of cellular stress responses. Phosphorylation at threonine 18 (Thr18) represents an early post-translational modification triggered primarily by ATM kinase following DNA double-strand breaks. This Transcription Factor Activity Assay from Assay Genie quantifies the DNA-binding capacity of Thr18-phosphorylated p53 captured from nuclear and cell lysates, supporting studies of pathway activation under genotoxic stress, oncogenic signaling, or pharmacological challenge.

The microplate workflow immobilizes a consensus p53 response element on the plate surface, with bound phospho-p53 revealed through an enzyme-substrate readout. Typical sample inputs fall in the 5 to 20 ug nuclear protein range per well. Antibody reagents show reactivity against human, mouse, and rat Thr18-phosphorylated p53, validating studies across common model systems.

Investigators studying apoptosis induction, cell-cycle checkpoint control, DNA damage response pathways, and cancer therapeutics development routinely apply this reagent. Store kit components refrigerated at 4 degrees C; aliquoted buffers intended for long-term stability should be kept frozen at -20 degrees C away from light. Reagents are intended for research use only in in vitro studies.