p53 (Phospho-Ser6)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit enables qualitative measurement of p53 phosphorylated at Serine 6 in cultured cells. The assay uses an indirect ELISA format in which p53 (Phospho-Ser6) is captured by phospho-specific primary antibodies and detected using HRP-conjugated secondary antibodies that catalyze a colorimetric reaction at 450 nm upon substrate addition. Described as lysate-free and high throughput, the kit supports relative quantification of phosphorylated p53 in cells and the screening of treatments, inhibitors such as siRNA or chemicals, and activators for their effects on p53 phosphorylation.

A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalizing target absorbance values. Following the colorimetric HRP measurement, the Crystal Violet whole-cell staining method may be applied to determine cell density, allowing absorbance values to be adjusted to cell amounts and correcting for plating differences. The recommended sample type is adherent cells, and the kit is described as offering high sensitivity and specificity for reliable and reproducible results.

The stated dynamic range is more than 5000 Cells. Associated database references include Gene ID 7157, UniProt ID P04637, and Gene Symbol TP53, with the sub type listed as Phospho. Reactivity is indicated for human samples.