p53 (Phospho-Ser366)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the accurate detection of phosphorylated p53 protein levels in cell lysates. It targets p53 phosphorylated at Ser366, a key biomarker in cell cycle regulation, DNA damage response, and apoptosis — pathways whose dysregulation has been implicated in cancer and neurodegenerative disorders.

The assay uses an indirect ELISA format in which p53 (Phospho-Ser366) is captured by target-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction read at 450 nm. The kit is described as lysate-free and high throughput, enabling measurement of relative phosphorylated p53 levels in cultured cells and screening of the effects of treatments, inhibitors (e.g. siRNA or chemicals), or activators on p53 phosphorylation. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalization, and a Crystal Violet whole-cell staining method can be used to determine cell density and adjust for plating differences.

The kit is reactive with human samples, with a stated dynamic range of > 5000 Cells, and the target is catalogued under Gene ID 7157 and UniProt ID P04637 (Gene Symbol: TP53).