p53 (Phospho-Ser20)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit is intended for the detection and quantification of phosphorylated serine 20 levels of the p53 protein in cell lysates. The assay targets p53 (Phospho-Ser20) with reactivity in human, mouse, and rat samples, and is suitable for use with adherent cells. Detection is colorimetric at 450 nm, with a stated dynamic range of > 5000 Cells.

The kit uses a qualitative indirect ELISA format in which p53 (Phospho-Ser20) is captured by phospho-specific primary antibodies and detected with HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control to normalize target absorbance, and Crystal Violet whole-cell staining may be used to determine cell density and adjust for plating differences. The kit supports research into p53 signalling, the DNA damage response, and cancer biology, where serine 20 phosphorylation regulates p53 stability and activity, with dysregulation linked to tumorigenesis and resistance to cancer therapies.