p50 Dynamitin Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA kit is designed for the detection of p50 Dynamitin in adherent cells, with reactivity across Human, Mouse, and Rat. The assay uses a colorimetric detection method measured at 450 nm and follows an indirect ELISA format in which the target protein is captured by p50 Dynamitin-specific primary antibodies and detected using HRP-conjugated secondary antibodies that bind the Fc region of the primary antibody.

As a lysate-free, high-throughput and sensitive solution, the kit enables qualitative measurement of p50 Dynamitin protein expression profiles in cultured cells. It can be used to determine the relative amounts of p50 Dynamitin and to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on the protein. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalising target absorbance values, while a Crystal Violet whole-cell staining step can be applied to determine cell density and adjust for plating differences. The kit is described as offering high specificity and reproducibility.

The target, p50 Dynamitin (gene symbol DCTN2), is a 50 kDa subunit of the dynactin complex that is essential for dynein motor function and microtubule organisation, with dysregulation implicated in neurodegenerative disorders and cancer. The kit is supplied in a 96-well microplate format with a dynamic range of > 5000 cells and a storage stability of 6 months at 4°C.