p300 Colorimetric Cell-Based ELISA Kit

Assay Genie's cell-based ELISA kit targets p300 (EP300), a histone acetyltransferase and transcriptional co-activator that regulates transcription via chromatin remodelling and plays roles in gene expression, cell growth and differentiation. The kit is a lysate-free, high-throughput indirect ELISA designed to detect p300 protein expression directly in cultured cells, making it suitable for measuring relative p300 levels and screening the effects of treatments, inhibitors or activators.

Detection is achieved by an indirect ELISA format in which p300 is captured by target-specific primary antibodies and detected with HRP-conjugated secondary antibodies that catalyse a colorimetric reaction measured at 450 nm. The stated dynamic range is greater than 5000 cells. A monoclonal anti-human GAPDH antibody is included as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining method may be used to determine cell density for additional normalisation. Reactivity is given for Human, Mouse and Rat samples, with adherent cells as the intended sample type, in a 96-Well Microplate format. Storage stability is stated as 4 °C for 6 months.

The kit supports research into p300's involvement in processes such as transcription, the DNA damage response, hypoxia-induced gene regulation and cell-cycle progression, with associated database identifiers including Gene ID 2033 and UniProt ID Q09472.