p300/CBP Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit targets the p300/CBP complex, key transcriptional coactivators that interact with a wide range of transcription factors to regulate gene expression. The assay uses a colorimetric detection method at 450 nm and is configured in a 96-well microplate format described as high throughput.

Reactive with human, mouse, and rat samples, the lysate-free indirect ELISA captures p300/CBP via specific primary antibodies, with HRP-conjugated secondary antibodies enabling the colorimetric signal. The kit can be used to measure relative p300/CBP levels in cultured cells and to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators, with a stated dynamic range of > 5000 Cells. The sample type is adherent cells.

For data normalization, the kit includes an anti-GAPDH antibody as an internal positive control and supports the Crystal Violet whole-cell staining method for determining cell density. Storage is at 4°C with a stated stability of 6 Months. Database entries for the associated protein (Gene ID: 8850; UniProt ID: Q92831; Gene Symbol: KAT2B) note its function as a histone acetyltransferase that promotes transcriptional activation and participates in chromatin remodeling.