p27 Kip1 (Phospho-Thr198)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit provides a convenient, lysate-free, high throughput and sensitive assay for the qualitative detection of p27 Kip1 protein phosphorylation at threonine 198 in cultured human cells. The indirect ELISA format uses p27 Kip1 (Phospho-Thr198)-specific primary antibodies together with HRP-conjugated secondary antibodies to catalyze a colorimetric reaction at 450 nm, with a dynamic range of > 5000 Cells. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control, and Crystal Violet whole-cell staining may be used to determine cell density and normalize absorbance values.

Intended for adherent cell samples, the kit supports research into p27 Kip1 (CDKN1B), a cyclin-dependent kinase inhibitor that regulates cell cycle progression at G1 and acts as a tumor suppressor by inhibiting cyclin-dependent kinase activity. Phosphorylation of p27 Kip1 at threonine 198 has been shown to modulate its function, impacting cell proliferation and apoptosis, and its dysregulation has been implicated in cancer, neurodegenerative disorders, and multiple endocrine neoplasia type IV. The kit can be used to measure relative phosphorylation levels in cultured cells and to screen the effects of various treatments, inhibitors, or activators on p27 Kip1 phosphorylation.