OXSR1 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit is designed for the qualitative measurement of oxidative stress responsive kinase 1 (OXSR1) in cell lysates and tissue homogenates. The assay is intended for use with adherent cells and can be applied to determine the relative amounts of OXSR1 in cultured cells as well as to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on OXSR1 expression.

The assay uses an indirect ELISA format in which OXSR1 is captured by target-specific primary antibodies and detected via HRP-conjugated secondary antibodies. Substrate addition produces a colorimetric reaction read at 450 nm. Because the assay is qualitative, two normalization strategies are provided: a monoclonal anti-GAPDH antibody serves as an internal positive control, and Crystal Violet whole-cell staining can be used to assess cell density and adjust for plating differences. Stated reactivity covers human and mouse samples, with a dynamic range of greater than 5000 cells. Storage is at 4°C for 6 months, and the kit is supplied in a 96-well microplate format.

Included components are a 96-well cell culture clear-bottom microplate, 10X TBS, quenching buffer, blocking buffer, 15X wash buffer, primary antibody diluent, 100X anti-phospho target antibody, 100X anti-target antibody, anti-GAPDH antibody, HRP-conjugated anti-rabbit IgG antibody, HRP-conjugated anti-mouse IgG antibody, SDS solution, stop solution, ready-to-use substrate, crystal violet solution, and adhesive plate seals. Equipment and reagents required but not supplied include a microplate reader capable of measuring absorbance at 450 nm (and optionally 595 nm for Crystal Violet staining), micropipettes, 37% formaldehyde, and a wash apparatus such as a manifold dispenser, multichannel pipette reservoir, or automated plate washer.