OTA (Ochratoxin A) ELISA Kit

Food safety laboratories monitoring cereal and feed contamination rely on rapid, quantitative screening for Ochratoxin A, a nephrotoxic mycotoxin produced by Aspergillus ochraceus, Aspergillus carbonarius, and Penicillium verrucosum. Regulatory bodies including the European Commission have set maximum permitted levels for OTA in foodstuffs at 5 µg/kg for raw cereals and 3 µg/kg for processed cereal products.

The Assay Genie Ochratoxin A ELISA Kit provides a competitive immunoassay workflow optimized for grain and feed matrices, enabling labs to screen samples against regulatory thresholds without the cost of LC-MS instrumentation. The kit runs on a standard microplate, supports 96 assay determinations per run, and produces a colorimetric readout compatible with standard plate readers.

Sample preparation involves extraction of ground grain or feed with an aqueous methanol mixture, followed by dilution and clean-up before loading onto the antibody-coated plate. Common applications include routine mycotoxin monitoring in milling operations, feed quality control at grain elevators, and academic research on fungal contamination. Reagents should be kept refrigerated between uses and brought to room temperature before each run, with the conjugate and standards stored in individually sealed vials to preserve stability across multiple batches.