OCT2 Colorimetric Cell-Based ELISA Kit

A colorimetric cell-based ELISA kit from Assay Genie is designed to measure OCT2 protein directly in cultured cells using an indirect ELISA format. OCT2 is captured by target-specific primary antibodies and detected with HRP-conjugated secondary antibodies; the resulting HRP activity is read at 450 nm after substrate addition. The kit is provided in a 96-well microplate format and is intended for adherent cell samples, with a stated dynamic range of greater than 5000 cells.

Validated for human and mouse reactivity, the kit is described as a lysate-free, high-throughput assay for the relative quantification of Oct2 expression and for screening the effects of treatments, siRNA, or chemical inhibitors and activators. An anti-GAPDH antibody is included as an internal positive control to normalise target absorbance, and a Crystal Violet whole-cell staining step is provided so that readings can additionally be adjusted to cell density. Storage is at 4 °C with a stated stability of 6 months.

The associated gene is POU2F2 (Gene ID 5452; UniProt P09086; OMIM 164176). Source documentation describes the protein as a POU-domain transcription factor that binds the octamer motif 5'-ATTTGCAT-3' and is involved in immunoglobulin gene regulation and B-cell maturation, while marketing material also refers to OCT2 as an organic cation/carnitine transporter relevant to drug transport, metabolism, pharmacokinetics, and drug–drug interaction studies.